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Z70 Full Version Full Version.Biological activity of aortic extracts in experimental heart failure.
This study was conducted to investigate the hypothesis that aortic extracts, obtained from aortas of bilaterally nephrectomized or bilateral adrenalectomized rats, might be useful in the treatment of heart failure. Changes in the contractile and relaxation properties of spontaneously beating cardiomyocyte isolated from the left ventricle of sham-operated, bilateral adrenalectomized or bilaterally nephrectomized rats were compared. Cardiomyocyte isolated from bilaterally nephrectomized rats displayed both a greater decrease in twitch tension and a higher increase in the cAMP content compared to those isolated from sham-operated or bilateral adrenalectomized rats. Increases in twitch tension and myocardial cAMP content were associated with faster relaxation. In the presence of Gpp(NH)p (10 microM), no difference in the basal rates of relaxation could be observed between the different groups of cardiomyocytes isolated from intact rats. In preparations from bilaterally nephrectomized rats, atropine (1 microM) blocked the effects of Gpp(NH)p and increased relaxation time. We conclude that the lower twitch tension and higher cAMP content recorded in cardiomyocytes isolated from the left ventricle of bilaterally nephrectomized rats can be attributed to a decreased activity of